( ! ) Notice: Undefined index: paginaFinal in /var/www/html/portugal/modulos/meta-tags-esc.php on line 61 Call Stack #TimeMemoryFunctionLocation 10.0006243280{main}( ).../index.php:0 20.46203217976include( '/var/www/html/portugal/plantilla/cabecera.php' ).../index.php:44 30.46243237488include( '/var/www/html/portugal/modulos/meta-tags-esc.php' ).../cabecera.php:5 " name="citation_lastpage" /> Regulation of transcription factor MEF2C by RNA binding protein HuR
PS021
Regulation of transcription factor MEF2C by RNA binding protein HuR
Z. Anyu1, G. Shi1, A. Xie1, D. Aksoy2,, , S. Dudley1
1 Cardiovascular Research Center, The Warren Albert Medical School of Brown University, Providence Rhode Island, United States
2 Marmara University School of Medicine, Istanbul, Turkey

Aim: We hypothesized that HuR RNA binding protein regulates MEF2C expression through association with MEF2C mRNA.

Introduction: MEF2C is earliest expressed member of the MADS-box super family during heart development. In the postnatal heart, decreased expression of MEF2C has been associated with myotonic dystrophy type 1 (DM1) heart disease. Hu proteins are known to regulate a wide range of gene expression by modulating mRNA's half-lives.

Methods: We use Human Fetal Cardiomyocyte cell line RL14. Cells are transfected with Superfect Transfection Reagent(Qiagen). And RNA Isolation performed by using RNeasy Plus Mini Kit. Real Time quantitative PCR (q-PCR) analysis performed using Fast SYBR Green Master Mix.

Results: Over expression of HuR in cardiomyocytes derived from primary human fetal ventricle increased MEF2C mRNA 47.3% (p=0.01). Knocking down of HuR by siRNA decreased MEF2C mRNA by 62% (p=0.01). RNA Immunoprecipitation showed HuR associated with MEF2C mRNA.

Conclusion: Our results suggest that RNA binding protein HuR associates with MEF2C mRNA in cardiomyocytes. And also HuR positively regulates MEF2C mRNA expression.


( ! ) Warning: Invalid argument supplied for foreach() in /var/www/html/includes_ws/librerias/html/item.php on line 1203
Call Stack
#TimeMemoryFunctionLocation
10.0006243280{main}( ).../index.php:0
20.46333212312include( '/var/www/html/portugal/plantilla/central.php' ).../index.php:45
30.46423255800include( '/var/www/html/portugal/contenidos/item.php' ).../central.php:11
40.52403259616getAutorCorrespondenciaHTML( ).../item.php:281

Open Access

Creative Commons License
Porto Biomedical Journal is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

+ info