d-Galactose high-dose administration and oral epigallocatechin-3-gallatte effects on the dendritic trees of developing neurons of young male rats" name="citation_title" /> <span class="elsevierStyleSmallCaps">d</span>-Galactose high-dose administration and oral epigallocatechin-3-gallatte effects on the dendritic trees of developing neurons of young male rats
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d-Galactose high-dose administration and oral epigallocatechin-3-gallatte effects on the dendritic trees of developing neurons of young male rats
F. Barreto1,2,, , R. Rodrigues1,2, A. Cardoso1,2, J.P. Andrade1,2
1 Department of Biomedicine – Unit of Anatomy, Faculty of Medicine, University of Porto, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal
2 Center of Health Technology and Services Research (CINTESIS), Faculty of Medicine, University of Porto, Rua Dr. Plácido da Costa, 4200-450 Porto, Portugal

Aim: In the present study, we aimed to explore the effect of d-galactose administration and epigallocatechin-3-gallatte (EGCG) on the dendritic trees of developing granule cells of the hippocampal formation (HF) of young male rats.

Introduction: The model of accelerated senescence with the administration of d-galactose is used in anti-aging studies. However, reports have questioned its effectiveness. To clarify this issue we used high-dose d-galactose on young rats and studied the immature granule cells stained with the neurogenesis marker doublecortin (DCX). We also used EGCG, a green tea catechin, to verify if there are neuroprotective effects in the d-galactose-treated animals.

Methods: At 4 weeks of age, male Wistar rats were allocated to a control group (n=7), a d-galactose group (300mg/kg body weight, intraperitoneally) (n=5; GAL) and to a d-galactose+EGCG (oral solution, 2 grams/L) group (n=5; gal+EGCG) during 4 weeks. After this period DCX immunocytochemistry was performed. The dendritic trees of immature granule cells were drawn with the aid of a camera lucida and a metric analysis of the dendritic segments of the dendritic trees was performed.

Results: No differences in all parameters quantified were found when controls and gal rats were compared. However, the results show that the total dendritic length of the dendritic trees of gal+EGCG rats was significantly reduced when compared with controls (p<0.03). There were no differences in the others dendritic parameters quantified.

Conclusion:d-Galactose did not induce disturbance of the neurogenesis as shown by the absence of alterations in the dendritic trees confirming our previous studies. Surprisingly, the addition of EGCG led to a reduced total dendritic length. This unexpected effect can be explained if we consider that the addition of the catechin acted as a second aggression leading to a disturbed dendritic tree of the immature neurons.

Acknowledgements: This article was supported by ERDF through the operation POCI-01-0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização – COMPETE2020 and by National Funds through FCT - Fundação para a Ciência e a Tecnologia within CINTESIS, R&D Unit (reference UID/IC/4255/2013).

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